Hi there,
Yes you can as long as ESXi "sees" the two ports on the network adapter as seperate network adapters. This will allow you to create multiple vSwitches and connect each network adapter (port) to a different one.
Post a screenshot of the ESXi host Physical adapters page (ESXi host > Configure > Networking > Physical Adapters from the WebClient) or the model number of the network adapter you have - this would make it easier to confirm for certain.
Kind regards.